As soon as the system is connected to power, the inbuilt pump will generate a pre-set low and high pressure relative to the ambient pressure which can be anywhere between -0.8 bar and 1.5 bar. When the output pressure of the system reaches the pre-set deviation threshold, the pump is activated again and the pressure reservoirs are refilled automatically.
Depending on the cartridge materials, reagent reservoir type and reagent viscosity, lower or higher pressure ranges might be needed to efficiently enable reagent supply. To allow this, the pressure regulating system can be pre-programmed so that the output pressures change over time according to the requirements of your assay setup.